Present research reports the enhanced diabetic wound healing potential of copper nanoparticles (CuNPs) synthesized from Mentha piperita obtained from high-altitude areas. Medioresinol was identified as a novel metabolite in M. piperita methanolic extract through Liquid chromatographic-tandem mass spectrometric analysis (LC-MS/MS). UV-vis and Fourier transform infrared spectroscopy confirm NPs with the presence of phenolic, amino, and ether groups. Spherical-shaped NPs with an average size of 100 nm were revealed by scanning electron microscopy. The bactericidal efficacy of CuNPs (2 mg/ml) against methicillin-resistant Staphylococcus aureus with an 18.3 mm inhibition zone was observed. CuNPs vividly possess antiglycation potential with 80% inhibition compared to M. piperita extract at 69%. Excision wounds were created on diabetic rats after receiving a single injection of alloxan monohydrate (150 mg/kg). One hour post diabetic induction, rats were treated with a topical formulation of CuNP-gel at 5-30mg/kg for 20 days and wound morphological parameters along with histopathological changes were evaluated. Conclusively, M. piperitabased CuNPs proved a critical role in wound epithelialization, proliferation, and differentiation with improved wound contraction in a dose-dependent manner, with 25 mg/kg as the best dose compared to positive and negative control groups. M. piperita metabolites enhanced the CuNP bactericidal and antidiabetic potential, along with wound healing activity in-vivo and in-vitro.