The parasitic herb Cuscuta reflexa is perennial, member of the “Convolvulaceae” family, golden yellow and leafless. Cuscuta reflexa, called dodder, is a holoparasite with a very low level of chlorophyll and photosynthesis activity. Qualitative and quantitative analysis on the methanol extract and its fractions were performed. Total phenolics content using Folin-Ciocalteu reagent and total flavonoid content by aluminium chloride assay was measured. Methanol extract was analyzed through gas chromatography-mass spectrometry. Biological activities including anti-oxidant, anti-diabetic, anti-inflammatory, anti-bacterial, hemolytic, and thrombolytic activities carried out at different concentrations of these extracts to measure the potential of each concentration. Qualitative analysis confirmed the presence of many biological compounds i.e. tannins, terpenoids, steroids, phenols, glycosides. Ethyl acetate fraction found to be had highest total phenolic (34 ± 0.026) and total flavonoid content (87 ± 0.014) value. Gas chromatographymass spectrometry confirmed the presence of many bioactive chemicals. Cuscuta reflexa has shown to have significant biological properties. Methanol extract and its ethyl acetate fraction showed significant zones of inhibition against gram positive (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae). Staphylococcus aureus was the most sensitive one than other bacteria used to test. It is concluded in this study that Cuscuta reflexa can be effectively used to develop novel drugs against bacterial infections, inflammation, diabetes, and oxidation.