MicroRNAs (miRNAs), an important biological tool are strong regulators of gene expression; functioning in RNA silencing or controlling gene expression post-transcriptionally. It has been shown that besides the important role of miRNA in normal body functioning, abnormal expression of miRNA also has role in many serious diseases like cancer. The abnormal expression of miRNAs have been proposed to act as oncogene either by up-regulation of oncogenic mRNA or down-regulation of tumor suppressor genes in most malignancies such as in breast cancer (BC). Considering the important role of miRNAs, the present study was performed to screen the collected BC samples for known mutation in candidate miRNA genes and to establish co-relation of these genetic variations in miRNA genes with BC in the selected patients of District Swat, Pakistan. Blood samples from BC patients were collected at SINOR cancer hospital Swat and were then amplified with polymerase chain reaction (PCR) using two sets of newly designed primers. The PCR products were sequenced and screened for the presence of SNPs in hsa-mir-381, hsa-mir-487b and hsamir-126. This study reports the presence of SNP rs10083406 A>C, downstream to hsa-mir-381 and upstream to hsa-mir-487b while in hsa-mir-126, SNP rs4636297 A>G and SNPrs1140713 C>T were found. The resulted SNPs were analyzed statistically and found no association of these SNPs with BC. The negative association of the studied SNPs with BC may be due to small number of sequenced samples, however large number of samples may be sequenced to study the association of these SNPs with BC and analyze its frequency in the studied region of District Swat, Pakistan.