Rhizopus oryzae is work as a model study for evaluation fungal evolution. It represents as a dangerous infection characterized by fast angioinvasive growth. Furthermore, Azole resistance R. oryzae isolates represent an economic problem. Green fabrication of silver nanoparticles is better than chemical methods, as it is environmental and cheap-cost. In the present study, azole resistance R. oryzae isolates were collected from maize grains fields that had been sprayed with azole fungicides and then isolated. Thenm applied silver nanoparticles were synthesized by biological methods, using Aspergillus niger as a reducer. Silver nanoparticles were characterized by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM), the antifungal activity of silver nanoparticles against Azole resistance R. oryzae isolates were collected from maize grains soil that had been remedied with azole fungicides was evaluated using the disc diffusion method, minimum inhibitory concentration (MIC). The physiochemical results emphasized the fabrication of Ag-NPs in spherical shapes with a diameter of 14 nm without any aggregation. MIC of Ag-NPs against azole resistance R. oryzae isolates from white and yellow maize was 62.5, 31.25 mg/mL, respectively. Finally, the goal of the report is utilize the silver nanoparticles as antifungal agents against Azole resistance Rhizopus oryzae isolates.