The aim of tillers is the high quality, productivity and gain from their crops. Positive effects of lime and biofertilizer application significantly improved the soil nutrient and raised plant yield. The main goal of this research found out impacts of lime, bio-organic combined with inorganic fertilizer on soil properties and chili yield. The study, which was designed in Chau Thanh district, An Giang province, Vietnam, consisted of five treatments of bio-fertilizer (1.0 ton Trichomix DT02/ ha), lime (3.0 tons CaCO3 /ha) and three inorganic fertilizer ratios of NPK:60-60-60 kg/ha were applied on soil properties and chili yield. As a result, the coapplication of 3.0 tons CaCO3 /ha + 1.0 ton Trichomix DT02/ha+NPK remarkably raised the soil chemical constituents and chili yield in all treatments. Conversely, the co-application of 3.0 tons CaCO3 /ha and/or 1.0 ton Trichomix DT02/ha had the lower yields. The highest chili yield was significantly observed under the added treatment of lime, Trichomix DT02 and NPK. The soil chemical constituents such as total nitrogen, available phosphorus, exchangeable potassium and organic matter, which were significantly impacted by amendment treatments of lime, Trichomix DT02 and/or NPK fertilizer in differently amended levels. The added treatment of Lime, Trichomix DT02 and NPK obtained the maximum chili yield, which raised from 37.6% at C1 (NPK alone); 45.5% at C2 (CaCO3 alone) and59.8% at C3 (Trichomix DT02 alone) and 23.5% at C4 when comparing to C5 (1.0 tons Trichomix DT02/ha + 3.0 tons CaCO3/ha + NPK). From this research results, Co- application of lime, bio-fertilizers and inorganic fertilizers, which need to be recommended to famers to promote soil nutrients and plant yield.