1. EMAN M. SHARAF - Bacteriology, Immunology and Mycology Department, Animal Health Research Institute, Shebin El Kom, Menofia (ARC).
2. SANAA ALMOWALLAD - Department of Biochemistry, Faculty of Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia.
3. FUAD A. ALATAWI - Department of Biology, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia.
4. MUNEEFAH ABDULLAH ALENEZI - Department of Biology, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia.
5. MAHA MOHAMMED ALHARBI - Department of Biology, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia.
6. FAUZEYA MATEQ ALBALWE - Department of Biology, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia.
7. WEDAD AHMED - Department of Bacteriology, Mycology and Immunology, Fac. Vet. Med, Benha University, Benha 13736,
Egypt.
8. DOAA BAHAA ELDIN DARWISH - Department of Biology, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia.
Botany Department, Faculty of Science, Mansoura University, 35511 Mansoura, Egypt.
9. AHMED S. GEBREIL - Botany Department, Faculty of Science, Mansoura University, 35511 Mansoura, Egypt.
E. coli is one of the key bacterial groups of Human microbiomes that present in the gastrointestinal tract; most isolates of it are non-pathogenic except if the host immune system is compromised then it may be considered as an opportunistic in those circumstances. Strains of E. coli can be involved in the pathogenesis operation of causing several diseases, such as colibacillosis in poultry and hemolytic uremic syndrome in humans. We aimed at evaluating and assessing the anti-bacterial action that resulted from using glycyrrhizic acid against E. coli bacteria which have been isolated from ready-to-eat meat products. The bacteria have been identified through utilization of both biochemical trials like oxidase, catalase, urease, citrate utilization and methyl red test, and polymerase chain reaction (PCR) that confirmed the identification through clarification of the band position on agarose gel. PCR was utilized for recognition of several resistance genes, for instance, ampC, TetA (A) and mphA in the isolates. The anti-bacterial ability of glycyrrhizic acid was then being evaluated at first through in vitro studies using agar well diffusion method. Obtaining of both MIC value and mean inhibition zone diameter has been done; values were 2500 µg/ml and 13 mm, respectively. Lastly, in silico studies have been made by using molecular docking to determine the binding mode and affinity of glycyrrhizic acid with gene expression products of both blaNDM gene and OptrA gene in E.coli.
E. Coli, Molecular Docking, Glycyrrhizic Acid, Licorice.