The Hepatitis C viral infection contributes to a significant health burden worldwide. In Pakistan, about 6% of individuals are affected by HCV. Acute hepatitis is usually asymptomatic and rarely causes symptoms such as jaundice, fatigue, fever, and nausea. Around 85% of people affected with acute HCV initially may develop chronic disease. This study aims to find the effect of HCV viral load in the de-arrangements of triglycerides levels, ALT levels, total cholesterol levels, uric acid levels, hemoglobin, and WBCs quantity. After the HCV diagnosis with q-PCR, patients' blood samples were collected in EDTA and Gel tubes to estimate hematological, biochemical, and lipid profile parameters. The estimation of hemoglobin (Hb) and WBCs (white blood cells) was done by the hematological analyzer and the estimation of triglycerides, cholesterol, uric acid, and ALT levels was done by micro lab 300. Simple linear regression and persons correlation show a significant positive correlation of viral load with triglycerides (p=0.008), ALT (p=<0.001), and WBCs (p<0.001). Uric acid (p=0.304) and total cholesterol (p=0.76) shows a non-significant positive correlation. However, hemoglobin (p=0.857) shows a non-significant negative correlation with HCV viral load.