1. GHULAM YASEEN - Pesticide Quality Control Lab, AARI, Faisalabad.
2. ZAFAR ABBAS - Soil and Water Testing Laboratory, Dera Ghazi Khan.
3. KHALIQ-UR-REHMAN ARSHAD - Pesticide Quality Control Lab, AARI, Faisalabad.
4. ZIA CHISHTI - Pulses Research Institute, Faisalabad.
5. ALI AFZAL - Provincial Pesticides Reference Laboratory, Kala Shah Kaku.
6. MUHAMMAD AWAIS KHALID - Pesticide Quality Control Lab, AARI, Faisalabad.
7. MUHAMMAD ADNAN RAFIQUE - Pesticide Quality Control Laboratory, Kala Shah Kaku.
A novel method of analysis of Novaluron is validated on reverse phase liquid chromatography using Infinity1260 series High Performance Liquid Chromatograph (HPLC) of Agilent Technologies equipped with UVVIS detector and Auto-Sampler. Eclipse C18 Column of dimension 250 x 4.6 mm, 5 µm is used. Sample is dissolved in acetonitrile. The composition of mobile phase used is ACN: H2O (65:35). 1.5 ml min-1 is flow rate and detection of Novaluron is carried out on 260 nm and at 8.22 min. Total run time of this chromatography is 10 min. This method is specific, accurate, precise and robust with LOD 7.6 ppm and LOQ 23.1 ppm. All the parameters of validation meet the given criteria. This study has been conducted at Pesticide Quality Control Laboratory Faisalabad as a requirement of ISO/IEC 17025.
Method validation, Novaluron, pesticide, High Performance Liquid Chromatography.