Burkholderia glumae is a bacterial infectious agent that is carried by seeds and can cause rice disease named Panicle Blight which as a result considerably decreases the yield and quality of rice all over the world. This particular research was carried out in order to isolate bacterial strain from infected rice seeds, characterize the morphology and to screen Pakistani rice varieties against bacterial panicle blight. The samples were isolated and purified by using King’s B agar medium. The morphological characters were studied by using Gram staining, Scanning electron microscope and other biochemical tests. The results showed that the isolated bacteria, was Gram negative. On the basis of morphology, bacteria had monotrichous rod shape. Screening of 14 Pakistani rice genotypes revealed that Super Basmati and Super Gold are most susceptible against bacterial panicle blight. This study will indicate and benefit in the selection of high yielding and best resistant Pakistani rice variety/varieties and may also help the plant breeders to select better rice genotypes to have promising rice lines with high yield potential and disease resistance and to enhance the food quality and quantity.