The Arecaceae family also known as Palmae, is a large group of plants belonging to the Arecales order. This research involved an anatomical study of transverse sections of roots from various species within the Arecaceae family. Anatomical studies were done through light and scanning electron microscope. Different anatomical features Epidermis cells thickness, cortex layers, cortex thickness, rhizodermis thickness, air spaces, vascular cylinder diameter, xylem, diameter of metaxylem, maximum length of phloem and pith diameter were examined. The epidermis cells thickness ranges from 17-61 µm. The cortex thickness ranges from 400-1040 µm. Rhizodermis thickness ranged between 13-35 µm. The maximum pith diameter observed was almost 820-990 µm in Nannorrhops ritchiana (Griff.) Aitch. Air spces ranged between 13-31 µm. Maximum length of phloem was seen in Dypsis lutescens (H.Wendl.) Beentje & J.Dransf. Dimeter of metaxylem ranged between 21-108 µm. In conclusion, the root transverse section cutting features exhibited significant variations, allowing for the differentiation of plants within the Arecaceae family. This is the first reported study on root section cutting of family Arecaceae species.