The Rutaceae family is a large group of flowering plants placed in the order Sapindales. This study involved an anatomical study of transverse sections of roots from selected species within the Rutaceae family. Light and scanning electron microscope was used for anatomical study. Different anatomical characteristics exodermis length and width, cork cells length and width, cortex parenchyma cells length and width, endodermis length, phloem strand length, xylem tracheae diameter, vessel length and width and pith cells diameter were examined. The exodermis cells length ranges from 47-145 µm. The exodermis cells width ranges from 24-78.5 µm. Cork Cells Length ranged between 46-120.5 µm. The maximum endodermis length observed was almost 110-120.5 µm in Citrus Sinensis. The maximum phloem strand length observed in Citrus Paradisi was 175-182 µm. Maximum pith diameter was seen in Citrus Grandis. In conclusion, the characteristics observed in the transverse sections of roots showed significant variations, enabling the differentiation of plants within the Rutaceae family. This is the first documented study on root section cutting in species of the Rutaceae Taxa.