Pneumonia is life threatening public health issue in developing countries. The emergence of resistance genes is an increasing challenge, lead to morbidity and mortality in Pakistani population. The purpose of this research was to assess potential risk factors, characterization, antibiogram and screening of resistance genes among clinical isolates of pneumonia. From November 2021 to January 2024; a cross-sectional study was carried out. Socio-demographic data was collected at tertiary care hospitals of Lahore, Punjab, Pakistan. From various clinical specimens, bacterial samples were collected. Bacterial strains were characterized on the basis of biochemical and molecular characterization. Antibiotic testing was performed using disk diffusion method. The frequency of β-lactamase (blaCTX-M, blaTEM and blaOXA) and macrolide resistant genes (erm (B) and mef (A)) in pneumonia isolates were screened and amplified by PCR. Data was analyzed statistically. Total of n=261 samples were collected and cultured, in which 128(49.0%) were positive. Pneumonia was more prevalent among pediatrics 133 (51.0%) and older adults 95 (36.4%). The dominant pathogens were K. pneumoniae (33.6%) and S. pneumoniae (21.8) while frequency of other isolates P. aeruginosa, S.aureus, E.coli and A.baumannii were less. The majority of bacterial strains showed resistance to penicillin, ampicillin and amoxicillin-clavulanate. Among all pneumonia isolates, the percentage of resistant genes blaCTX-M and blaTEM was greater than bla OXA while macrolide resistant genes mef (A) and erm(B) was more predominant in S. pneumoniae. It is concluded that β-lactam antibiotic is not first line drug for treatment of pneumonia. However, macrolide is better option for treating pneumonia in Pakistani population.