The present research aims to examine the effects of strategic leadership on strategic ambidexterity, as well as to test the mediating role of human resource capabilities in the relationship between these two concepts. A survey was conducted on a sample represented by (122) employees of several business hotels located in the city of Najaf, in Iraq. Analytical statistical tools were used, including correlation, regression (on the software SPSS V.28), as well as the structural equation modeling (SEM) approach and the Sobel test (on the software AMOS V.26). A set of conclusions were obtained, putting the emphasis on the strength of the mediating role of human resource capabilities in developing the association between strategic leadership and ambidexterity among employees of business hotels in the city of Najaf.