Background: Magnet hospital defined as an organization that is capable of attracting and retaining wellqualified nurses and then continually preparing them to deliver quality care. Aim: This study aimed at identifying the effect of magnet hospital Forces training program on nurse managers’ awareness of patient safety culture. Research design: A Quasi -experimental one group pretest posttest design was used. Setting: The study was conducted at Al-Hussien University Hospital in Cairo governorate affiliated to AlAzhar University. Subjects: All nurse managers working at the aforementioned study setting (50 nurse managers) were included in the study. Tools of data collection: Data were collected by using three tools, magnet hospital forces knowledge questionnaire, magnet hospital forces questionnaire and patient safety culture awareness questionnaire. Results: The present study revealed that there was high statistically significant relation between total nurse managers’ knowledge, perception about magnet hospital forces and their total awareness about patient safety culture pre, post, and follow-up program phase. As evidenced by, the nurse managers’ knowledge, perception about magnet hospital forces and their awareness about patient safety culture increased from (44%), (30%), (52%) pre-intervention to (82%), (62%), (84%) postintervention to (80%), (60%), (82%) at follow-up phase respectively. Conclusion: Implementing magnet hospital forces training program for nurse managers was effective in improving their awareness of patient safety culture, this confirmed the research hypothesis. Recommendations: The developed training program should be applied to all nurses in the study settings and in similar ones.