Venous thromboembolism (VTE), which includes deep vein thrombosis (DVT) and pulmonary embolism (PE), affects millions of people worldwide each year and has become the third most common vascular disease in most countries. VTE is regarded as a common cause of increased length of stay in hospitals and is a significant cause of morbidity and mortality in hospitalized patients, leading to high costs. Clinical nurses are key in preventing VTEs by identifying risks and applying preventive measures to enhance patient safety and reduce the occurrence and impact of VTEs. To assess Jordanian nurses’ level of perceived knowledge and practice of venous thromboembolism (VTE) risk assessment and prevention. A descriptive crosssectional study was conducted with ICU nurses using a predesigned questionnaire. Convenient sampling was used, and data were analyzed with SPSS 26, employing descriptive statistics, t-tests, and ANOVA with significance set at 0.05. The sample comprised 282 participants with a mean age of 30.6 years. Of the total sample, 55.3% were male, the majority (79.4%) had three or more years of experience working in the ICU, half reported attending a course about VTE, and 46.8 % of them self-rated their knowledge about VTE as good, while 25.1 % perceived their knowledge as very good or excellent. 49.5% was the average score for all VTE knowledge questions, out of 100. The average self-efficacy scores between 2.53 and 2.7 on a Likert scale of 1 to 5 for VTE prevention and prophylaxis. The most commonly cited barriers are "Lack of time" (17.0%) and "Lack of a standardized protocol" (16.3%). The self-reported VTE assessment performance of patients was inconsistent between nurses. Age and attending a course about VTE were the only statistically significant factors associated with knowledge level. The nurses in Jordan have limited knowledge and practice regarding VTE, particularly regarding risk factors and PE diagnosis. Time constraints and a lack of standardized protocols hinder their role in conducting thorough risk assessments and applying evidencebased prophylactic measures. Despite confidence, self-efficacy in VTE risk assessment and prophylaxis remains low. Educational and training programs targeting nurses working in the ICUs are needed, and including further VTE-related content in nursing programs is a must.