Introduction: - Gall Bladder is a viscous and hollow organ, located obliquely in the gall bladder fossa on the quadrate lobe of the liver. There are three parts of the gallbladder- the neck, the body, and the fundus. Histologically, the wall of the gall bladder is composed mucosa, muscularis externa, and adventitia or serosa. There is no submucosa or muscularis mucosae in the gallbladder wall. Aim & Objective: - To study of histopathological changes in the gallbladder mucosa of cholelithiasis. Material & Methods: - The current study was conducted in the anatomy department collaboration with the department of pathology and surgery, School of Medical Sciences & Research, Sharda University & Noida International Institute of Medical Sciences, Noida International University. A total of 140 specimens were selected from gallbladder after cholecystectomy. Results: - On gross examination was observed that outer gallbladder surface was congested in 100 specimens (71%), wall thickness was increased in 44 (31%) and mucosal abnormalities in 101 (72%). In microscopic finding maximum number of specimens had Chronic Cholecystitis132 (94%), followed by Rokitansky Aschoff sinuses 60 (42%), Muscular Hypertrophy 40 (28%), Epithelial Hyperplasia 15 (10%) Muscular gland metaplasia 10 (7%) and Intestinal Metaplasia 2 (1.4%). Conclusion: - In the present study it was concluded that Gallbladder disorders more prevalent in female. Chronic cholecystitis was the most frequent histological variation in the gallbladder mucosa associated with cholelithiasis, followed by rokitansky aschoff sinuses, muscular hyperplasia, epithelial hyperplasia, and intestinal metaplasia.