Background: Applying nursing care bundle on mechanically ventilated infants play a role in decreasing the incidence of ventilator associated pneumonia and improving their outcomes. Aim: To evaluate the effect of using nursing care bundle on developing ventilator associated pneumonia among infants. Design: A quasi experimental research pre/post-test design. Sample: A purposive sample of 100 infants on mechanical ventilator in the pediatric intensive care units aged ranges from 1-12 month divided equally into study and control groups. Setting: The study was carried out at pediatric intensive care units of two hospitals; El Mounira and specialized pediatric hospitals which affiliated to Cairo-University hospitals. Methods: Data collected using three tools including personal characteristics and medical history questionnaire, nursing care bundle and ventilator associated pneumonia indicator’s record. Results: three-fifths and l ess of infants in the study and control groups their age ranged from 6 to 12 months and from 1 to less than 6 months respectively, three-fifths and less in both groups were male. The main diagnosis was respiratory distress followed by neurological disorders. Three-fifths and less had oral endotracheal intubation, minority among both groups had unplanned extubation, mechanical ventilation days among the study group were less than the control group with significant difference. One fifth and more than one third of infants developed ventilator associated pneumonia among study and control groups respectively. Highly statistically significant negative correlations were detected between various components of the nursing care bundle and development of ventilator associated pneumonia. Conclusion: The current study concluded that application of the nursing care bundle significantly decreases the incidence of ventilator associated pneumonia among infants on mechanical ventilation as well as mean mechanical ventilation days, corticosteroids use and mortality. Recommendation: Application of nursing care bundle in pediatric intensive care units should be used as a routine care for infants and replication of the study on larger sample of infants in different settings.