Background: Healthcare-associated infections (HAIs) remain a major challenge worldwide, and healthcare workers’ (HCWs) adherence to infection prevention and control (IPC) recommendations is critical to reducing their incidence. Hand hygiene compliance and vaccination uptake are recognized as key preventive measures, yet sustaining improvements in these behaviors remains difficult. Objective: This systematic review aimed to evaluate the impact of dissemination and multimodal interventions on HCWs’ compliance with infection prevention and control recommendations for respiratory infectious diseases. Methods: The review was conducted in accordance with the PRISMA 2009 guidelines. A comprehensive search of Cochrane, Embase, and Medline databases was performed to identify randomized controlled trials (RCTs) published between 2010 and 2015. Studies assessing interventions designed to improve HCWs’ adherence to hand hygiene, vaccination uptake, or other IPC practices were eligible. Data were extracted independently by multiple reviewers and synthesized narratively due to heterogeneity in study designs and outcomes. Results: A total of six RCTs involving more than 54,000 HCWs were included. Interventions examined included the WHO multimodal hand hygiene strategy, distribution of educational materials, audit and feedback, vaccination campaigns, and workplace reminders. Educational interventions were common across studies, often combined with non-educational strategies such as feedback or infrastructure provision. Multimodal approaches demonstrated superior effectiveness compared with single interventions, with improvements in hand hygiene compliance ranging from 16% to 22% and significant increases in vaccination uptake. However, the evidence for direct reductions in infection incidence and MRSA colonization was less consistent. Conclusion: Multimodal dissemination strategies that integrate education, feedback, and organizational support are more effective than single interventions in improving HCWs’ compliance with IPC measures. While these interventions increase adherence to recommended practices, further research is needed to assess their long-term sustainability, cost-effectiveness, and direct impact on reducing HAIs in diverse healthcare settings.