1. MENNAT-ALLAH ABDELLATEF OTHMAN - Dentist, Medical Administration, Suez Canal University, Ismailia, Egypt.
2. SHAIMAA MOHAMED MAHFOUZ OMER - Professor of Pediatric Dentistry, Preventive Dentistry and Dental Public Health Faculty of Dentistry, Suez University, Suez, Egypt.
3. MERHAN NABIH ELMANSY - Associate Professor of Oral Pathology, Faculty of Dentistry, Suez Canal University Ismailia, Egypt.
Objectives: The study aimed to evaluate the topical anti-inflammatory effect of eucalyptus extract gel
compared with jojoba gel on 5-fluorouracil-induced OM in hamsters. Materials and Methods: Sixty male
hamsters were divided into two main groups. Group A (n = 15) served as a negative control and did not
receive OM induction or treatment. Group B (n = 45) underwent OM induction with two intraperitoneal
injections of 5-FU (60 mg/kg) followed by superficial scratching of the left cheek pouch. On day 5, Group B
was subdivided into three subgroups (n = 15 each): BΙ (positive control, untreated), BΙΙ (treated daily with
JJ gel), and BΙΙΙ (treated daily with 2% EC extract gel). Five animals per subgroup were euthanized on days
5, 9, and 14. Macroscopic, histological, and immunohistochemical analyses were performed. Results:
Macroscopic and histological evaluations revealed significant differences in re-epithelization and collagen
deposition among groups. Immunohistochemical analysis showed that EC gel treatment significantly
decreased interleukin-1 beta (IL-1β) expression and increased transforming growth factor-beta (TGF-β)
expression compared with JJ gel. Conclusions: Eucalyptus gel demonstrated superior promotion of ulcer
re-epithelization and accelerated healing compared with jojoba gel in the 5-fluorouracil induced oral
mucositis model.
Eucalyptus, Hamster, Jojoba, 5-Fluorouracil, Oral Mucositis.