Background: Evidence-based practice (EBP) is essential for improving healthcare quality by integrating the best available research evidence, clinical expertise, and patients' preferences into clinical decisionmaking. Nurse Managers play a key role in promoting EBP through leadership, research utilization, and quality improvement initiatives. However, inadequate EBP knowledge, limited implementation skills, and organizational barriers continue to hinder its integration into nursing management. Aim: to explore nurse managers’ knowledge, practices, attitudes, and barriers toward evidence-based practice Methods: descriptive exploratory design used in current study; at one of MOH Hospital, Jeddah. This study was conducted among (50) nurse managers. A purposive sample is used to recruit all nurse managers. Data were collected by using a structured questionnaire assessing EBP knowledge, practices, attitudes, and perceived barriers. Two tools were utilized, namely: (1) Personal Data Sheet and (2) Evidence-Based Practice Questionnaire (EBPQ), which included four parts: knowledge test, practice scale, attitude scale, and perceived barriers to evidence-based practice implementation. Results: Most nurse managers were female (84.0%), held a master's degree (62.0%), and had more than eight years of experience (88.0%). Overall, 66.0% demonstrated correct evidence-based practice knowledge, 42.0% had low practice, and 68.0% had negative attitudes. Heavy workload (86.0%) and limited time to read research (84.0%) were the main perceived barriers. Knowledge was significantly associated with most personal characteristics, whereas practice was associated only with educational level and previous research participation (p < 0.05). Conclusion: Nurse Managers demonstrated moderate knowledge but low practice and negative attitudes toward evidence-based practice. Organizational and individual barriers remain major challenges, highlighting the need for continuous education and organizational support to enhance evidence-based practice implementation. Recommendations: training program should be conducted for nurse managers to enhance their knowledge, practices, attitude related to Evidence Based Practice.