Objectives: The study aimed to evaluate the clinical and radiographic outcomes of human amniotic membrane compared to biodentine in pulpotomy of immature first permanent molars with pulp exposure. Materials and Methods: A randomized clinical trial was conducted on 50 immature permanent mandibular first molars requiring vital pulp therapy. Following pulp tissue amputation and hemostasis using saline, teeth were assigned to treatment with either human amniotic membrane (Group I) or biodentine (Group II). All teeth have received the same restorative protocol. Clinical examinations were done at 7 days, 3, 6, and 12 months while periapical radiographic examinations were used to monitor continued root development and detect possible pathological changes at 3, 6 and 12 months. Results: At 12 months, both materials demonstrated a 100% overall clinical success rate and a 100% radiographic success rate with complete symptom resolution and no pathological changes. No statistically significant differences were found between the two groups (p=1.000). Conclusions: Human amniotic membrane and biodentine are highly effective, bioactive materials for vital pulp therapy of immature first permanent molars.