Herbal medicine is experiencing a significant resurgence as natural approach to health and wellness. Gum Arabic, derived from the sap of Acacia trees, is gaining attention due to its numerous health benefits. This study investigates the protective effects of Gum Arabic against histopathological and physiological alterations induced by melamine toxicity in Swiss albino mice. Melamine exposure has been linked to various toxicological effects, particularly in kidney. The research involved administering Gum Arabic to mice subjected to melamine dose-1 and dose-2 according to their body weight, followed by assessments of physiological parameters and histological changes in kidney tissues. Results indicated that Gum Arabic significantly mitigated the adverse effects of melamine, evidenced by improved histopathological outcomes and restored physiological functions i.e weight gain. The findings suggest that Gum Arabic may serve as a potential therapeutic agent in counteracting melamine-induced toxicity, highlighting its role in enhancing health and reducing oxidative stress in affected tissues.