The bacterial isolate, identified as Pediococcus acidilactici (NMCC-G, MK072824, 97.65% similarity) with the sequences of different lactic acid bacteria species in the NCBI genes database was used for the production of novel probiotics. A biological trial was conducted by using one-day-old Ross-308 broiler birds to determine the supplemental effects of novel indigenous probiotics. The broiler chicks were allocated at random to one of the five dietary treatment groups in a way that each treatment group was fed to 5 replicates with 15 birds per replicate. Dietary treatments include probiotic-free basal diet (Control; C), indigenous probiotic (2.01 x 109 CFU/g) with three different inclusion levels; 1 gm/10 kg of diet (IProb-1), 1.5 gm/10 kg of diet (IProb-2) and 2.0 gm/10 kg of diet (IProb-3). The broiler diets were formulated according to nutrient specifications for Ross-308. The results indicated that broilers fed diets supplemented with IProb-3 had lower feed intake, higher (P<0.05) body weight gain, and better FCR in comparison to broilers fed control (no probiotics). Carcass yield and breast meat yield increased with increasing levels of IProb. The highest values were noted in IProb-3 (@ 2 gm/10 kg of diet). The total tract apparent digestibility coefficient for crude protein and dry matter was higher in probiotic-supplemented groups compared to the control. In conclusion, the locally isolated Pediococcus acidilactici NMCC-G strain @ 2.0 gm/10 kg (having 2.01 x109 CFU/g) resulted in better growth and feed efficiency in broiler birds as compared to that of nonsupplemented birds.