This study examines the factors influencing the quality of human resources (HR) in travel and tourism enterprises in Can Tho city, Vietnam. A survey of 350 employees from these enterprises was conducted, and data were analyzed using reliability testing, exploratory factor analysis (EFA), confirmatory factor analysis (CFA), and structural equation modeling (SEM) via SPSS 26.0 and AMOS 24.0. The results confirm that all 16 proposed hypotheses are supported at a 5% significance level, indicating positive relationships between HR quality and factors such as local government policies, linkages with training institutions, working environment, employee benefits, recruitment systems, training, digital transformation, and performance evaluation. The findings suggest that to enhance HR quality, enterprises should focus on improving internal factors like working conditions, benefits, recruitment, training, and digital adoption, while leveraging external support from local authorities and training institutions. These insights provide valuable recommendations for businesses and policymakers to improve HR management and contribute to the sustainable growth of the tourism sector in Can Tho city.