Background: The amniotic membrane, the inner layer of the placenta, possesses antibacterial properties that foster the healing process; however, its effect on cesarean section wound pain and healing remains underexplored. Aim: This study evaluates the effectiveness of prompt nursing intervention regarding amniotic membrane application on cesarean section wound pain and healing. Research Design: A quasiexperimental research design (study /control group) was adopted. Setting: The study was conducted at the labor unit of three main hospitals in Tanta, Egypt. Sample: A purposive sample of 60 laboring women was divided into two groups with each group comprising 30 women Tools for data collection: Three tools were used to collect data; Tool (I): Structured interviewing questionnaire. Tool (II): REEDA scale to assess the process of cesarean section wound healing. Tool (III): Laboring women's pain assessment questionnaire. Results: the study group's pain intensity decreased with a Mean ±SD of (9.07±2.27) compared to (12.2±4.01) in the control group, and the wound healing after seven days in the study group showed highly significant improvement (P value < 0.000). Conclusion: Applying an amniotic membrane to cesareansection wounds improves healing and reduces pain more than routine treatment, which validates the research hypothesis. Recommendations: Using amniotic membranes to treat cesarean section wound pain and healing is advised as a successful strategy for laboring women.